Hybridoma Services

Hybridoma Antibody Development

Phase I: Antigen preparation and Immunization

blue_arrow-lt Antigen preparation/Peptide Conjugation
blue_arrow-lt A maximum group of 5 mice will be immunized over a period of 10 weeks. Mouse strain used is BALB/c.
blue_arrow-lt Serum is screened by ELISA
blue_arrow-lt Highest titer screen will be selected for phase II

Estimated completion time: 12 weeks

*Price: $1,800.00

Phase II: Fusion and Selection

blue_arrow-lt Fusion of splenocytes to myeloma cell line
blue_arrow-lt All splenocytes will be used for fusion process
blue_arrow-lt Incubation in HAT (hypoxanthine-aminopterin-thymidine) selection media plated in 96 wells up to 10 plates per fusion.
blue_arrow-lt ELISA to confirm positive antibody

Estimated completion time: 2 weeks

*Price: $6,300.00

Phase III: Screening/Expansion/Cryopreservation

blue_arrow-lt Antibody Characterization to confirm Monoclonal antibody
blue_arrow-lt Isotype confirmation
blue_arrow-lt Cell Culture expansion up to the 10 best clones specific to antigen
blue_arrow-lt 3 vials per clone will be frozen and stored in LN2 (Liquid Nitrogen) until shipment

Estimated completion time: 8 weeks

*Price: $8,900.00

*Price list valid through December 31, 2016. 

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Hybridoma Development